PROTEIN LADDER WESTERN BLOT BIO RAD PLUS

The ChemiDoc MP Imaging System is an instrument for imaging and analyzing gels and western blots. We offer a blue prestained protein standard, as well as a colored prestained protein standard with multi-colored. Sizes range from 10 to 250 kDa which is ideal for accurate molecular weight determination for a wide range of expressed proteins. Additional data should be considered for a more accurate adjustment.Īpproximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25☌), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). Experience Bio-Rads New ChemiDoc MP - One System. New England Biolabs offers a selection of highly pure protein standards. Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. 2.5 μl per well for general Western transferring. Incubate the blot in the primary antibody and blocking buffer solution at 4☌ overnight with gentle agitation. Refer to the Blu11 (BlueAQUA) Prestained Protein Ladder patterns in various electrophoresis conditions:ģ μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Dilute the primary antibody 1:1,000 in 10 ml blocking buffer.

Guide for Molecular Weight Estimation (kDa) The Blu11 Prestained Protein Ladder (BlueAQUA) keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

PROTEIN LADDER WESTERN BLOT BIO RAD PLUS

The 11 recombinant proteins are covalently coupled with blue chromophore, and 2 reference bands at 25 kDa and 72 kDa serve as reference bands. Use improved Precision Plus Protein Dual Color Standards for molecular weight estimation on SDS-PAGE gels and western blots. Precision Plus Protein Kaleidoscope Standards are a mixture of ten multicolored recombinant proteins (10250 kD). The Blu11 Prestained Protein Ladder (BlueAQUA) is a combination of 11 pre-stained proteins with molecular weights from 10 to 180 kDa. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple.

Protein ladder western blot bio rad